Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Phusion Taq


  • Please log in to reply
2 replies to this topic

#1 gradstudent1

gradstudent1

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 14 December 2010 - 08:49 AM

I just wanted to know if storing the Phusion high fidelity Taq mix at -70 rather than the preferred -20 makes a difference in terms of product yield etc. Any experience/advice would be appreciated

#2 UBClabbie

UBClabbie

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 65 posts
8
Neutral

Posted 31 January 2011 - 04:44 PM

I just wanted to know if storing the Phusion high fidelity Taq mix at -70 rather than the preferred -20 makes a difference in terms of product yield etc. Any experience/advice would be appreciated



I don't think freezing and thawing enzymes or proteins from such a drastic range of temperatures (ie. -70 all the way to room temp) is good. I think it would just emphasize enzyme inactivity due to freeze-thaw. -70 is really meant for preserving cells, DNA, and RNA. Proteins should be kept at -20.

#3 stagius24

stagius24

    member

  • Active Members
  • Pip
  • 13 posts
0
Neutral

Posted 21 February 2011 - 09:36 PM

I hope they teach you that storing enzyme/protein at -70 or so is actually bas as they may start unfolding.
Unlike DNA, Protein is not "the colder, the better"




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.