Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Calculating


  • Please log in to reply
3 replies to this topic

#1 noyara

noyara

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 59 posts
1
Neutral

Posted 14 December 2010 - 08:48 AM

Good Day,

In Bugos et al. (1995)article, he extracted RNA as follow:
Tissue was ground to a fine powder in liquid nitrogen and added 1:2 (w/v) to 25:24:1
phenol:chloroform:isoamyl alchohol and vortexed.


My question is:
If i started with 0.1 g from plant tissue (leaf) how much i should add from phenol:chloroform:isoamyl alchohol?

0.2L or 0.2ml or what from P:C:I?

if 200ml, it's consider too high. isn't it?

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,516 posts
371
Excellent

Posted 14 December 2010 - 04:47 PM

He used 1:2 weight per volume - as you have 0.1 g use 0.2 ml.

#3 noyara

noyara

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 59 posts
1
Neutral

Posted 14 December 2010 - 06:51 PM

He used 1:2 weight per volume - as you have 0.1 g use 0.2 ml.


0.2ml not 0.2L?
but in calculating normally we used gram with litter, so when I used 0.1g then I should use 0.2L (200ml)
is it true?, or up to us, we can chose any unite as we want?

Please can you illustrate a littel bit more?
many thanks in advance

#4 Micro

Micro

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 60 posts
4
Neutral

Posted 14 December 2010 - 08:03 PM

I think you might be confusing using molecular weight in calculations (i.e. g/L) with ratios.

In ratios weight and volume need to be equivalant measurements:
kg=L
g=mL
mg=uL




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.