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# Calculating

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### #1 noyara

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Posted 14 December 2010 - 08:48 AM

Good Day,

In Bugos et al. (1995)article, he extracted RNA as follow:
Tissue was ground to a fine powder in liquid nitrogen and added 1:2 (w/v) to 25:24:1
phenol:chloroform:isoamyl alchohol and vortexed.

My question is:
If i started with 0.1 g from plant tissue (leaf) how much i should add from phenol:chloroform:isoamyl alchohol?

0.2L or 0.2ml or what from P:C:I?

if 200ml, it's consider too high. isn't it?

### #2 bob1

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Posted 14 December 2010 - 04:47 PM

He used 1:2 weight per volume - as you have 0.1 g use 0.2 ml.

### #3 noyara

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Posted 14 December 2010 - 06:51 PM

bob1, on 14 December 2010 - 04:47 PM, said:

He used 1:2 weight per volume - as you have 0.1 g use 0.2 ml.

0.2ml not 0.2L?
but in calculating normally we used gram with litter, so when I used 0.1g then I should use 0.2L (200ml)
is it true?, or up to us, we can  chose any unite as we want?

Please can you illustrate a littel bit more?

### #4 Micro

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Posted 14 December 2010 - 08:03 PM

I think you might be confusing using molecular weight in calculations (i.e. g/L) with ratios.

In ratios weight and volume need to be equivalant measurements:
kg=L
g=mL
mg=uL