Posted 14 December 2010 - 08:48 AM
In Bugos et al. (1995)article, he extracted RNA as follow:
Tissue was ground to a fine powder in liquid nitrogen and added 1:2 (w/v) to 25:24:1
phenol:chloroform:isoamyl alchohol and vortexed.
My question is:
If i started with 0.1 g from plant tissue (leaf) how much i should add from phenol:chloroform:isoamyl alchohol?
0.2L or 0.2ml or what from P:C:I?
if 200ml, it's consider too high. isn't it?
Posted 14 December 2010 - 04:47 PM
Posted 14 December 2010 - 06:51 PM
He used 1:2 weight per volume - as you have 0.1 g use 0.2 ml.
0.2ml not 0.2L?
but in calculating normally we used gram with litter, so when I used 0.1g then I should use 0.2L (200ml)
is it true?, or up to us, we can chose any unite as we want?
Please can you illustrate a littel bit more?
many thanks in advance
Posted 14 December 2010 - 08:03 PM
In ratios weight and volume need to be equivalant measurements: