How stable are the A-overhangs of PCR products? The pGEM-T easy protocol requires that PCR product are purified first, but I'd expect that to eliminate a lot of the T-overhangs. I was made to believe they were pretty delicate. Could that just be a fear instilled by Invitrogen and their pTOPO vector (because I think you don't need to purify the PCR product for that - I don't see any mention of it in their protcol, but it's been over a year since I did it).
Edit: Should be A-overhangs!
Edited by seanspotatobusiness, 14 December 2010 - 06:00 AM.