cell culture clarification
Posted 13 December 2010 - 11:28 AM
journal says "medium...was collected and clarified at 3000rpm in a Sorvall RT6000 refrigerated centrifuge for 10min at 4C." I do not know what is meant by "clarified" Do I use a special centrifuge tube with a filter? or am I over-analyzing this and it simply means to spin down? Or is there something I put in the centrifuge tube?
Thanks for any help
Posted 13 December 2010 - 02:24 PM
or am I over-analyzing this and it simply means to spin down?
In this context "clarification" means "removal of bigger debris" - eg. cells, cell fragments etc.
Posted 13 December 2010 - 03:00 PM
I was also wondering what they meant by 'adjusting the supernatant to...' but then realized they just meant added