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cell culture clarification


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#1 Dawn M

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Posted 13 December 2010 - 11:28 AM

I am a chemist who has gotten roped in to doing cell culture at work. I have to purify media collected from cells and I have never done it before. I have a protocol from a journal to work with. The cells have grown and media has been collected which I now need to purify.

journal says "medium...was collected and clarified at 3000rpm in a Sorvall RT6000 refrigerated centrifuge for 10min at 4C." I do not know what is meant by "clarified" Do I use a special centrifuge tube with a filter? or am I over-analyzing this and it simply means to spin down? Or is there something I put in the centrifuge tube?


Thanks for any help

#2 K.B.

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Posted 13 December 2010 - 02:24 PM

or am I over-analyzing this and it simply means to spin down?


That. :)

In this context "clarification" means "removal of bigger debris" - eg. cells, cell fragments etc.

#3 Dawn M

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Posted 13 December 2010 - 03:00 PM

haha :) thanks! I thought so. it just seemed too easy

I was also wondering what they meant by 'adjusting the supernatant to...' but then realized they just meant added :)




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