2 replies to this topic

[Dawn M]

I am a chemist who has gotten roped in to doing cell culture at work.  I have to purify media collected from cells and I have never done it before.  I have a protocol from a journal to work with.  The cells have grown and media has been collected which I now need to purify.

journal says "medium...was collected and clarified at 3000rpm in a Sorvall RT6000 refrigerated centrifuge for 10min at 4C."  I do not know what is meant by "clarified" Do I use a special centrifuge tube with a filter? or am I over-analyzing this and it simply means to spin down?  Or is there something I put in the centrifuge tube?


Thanks for any help

[K.B.]

Dawn M, on 13 December 2010 - 11:28 AM, said:

or am I over-analyzing this and it simply means to spin down?

That.

In this context "clarification" means "removal of bigger debris" - eg. cells, cell fragments etc.

[Dawn M]

haha thanks! I thought so.  it just seemed too easy

I was also wondering what they meant by 'adjusting the supernatant to...' but then realized they just meant added