5 replies to this topic

[noyara]

Hi,

How can I prepare:   phenol: chloroform: iso_amyal alcohol --> 25:24:1,   From 5:1 phenol:chloroform?

Thank you
Noor

[phage434]

You can't, if you really insist on the exact amounts.  I suppose you could evaporate some chloroform and carefully weigh things to reduce the amount of chloroform before adding isoamyl alcohol.  Or you could add a bit more phenol.  But that would be stupid.  Just add 2% isoamyl alcohol to get to 25:25:1 (well, not exactly, but close enough!).  And, just why is this important.  Usually phenol/chloroform will work just as well.  Major point -- be careful working with this.

[noyara]

phage434, on 12 December 2010 - 08:03 AM, said:

You can't, if you really insist on the exact amounts.  I suppose you could evaporate some chloroform and carefully weigh things to reduce the amount of chloroform before adding isoamyl alcohol.  Or you could add a bit more phenol.  But that would be stupid.  Just add 2% isoamyl alcohol to get to 25:25:1 (well, not exactly, but close enough!).  And, just why is this important.  Usually phenol/chloroform will work just as well.  Major point -- be careful working with this.

Thank you for your suggestion.

[mdfenko]

noyara, on 12 December 2010 - 06:13 AM, said:

Hi,

How can I prepare:   phenol: chloroform: iso_amyal alcohol --> 25:24:1,   From 5:1 phenol:chloroform?

Thank you
Noor

add 3.8 chloroform and 0.2 isoamyl alcohol. that should give you the proper ratios.

[noyara]

mdfenko, on 13 December 2010 - 09:13 AM, said:

noyara, on 12 December 2010 - 06:13 AM, said:

Hi,

How can I prepare:   phenol: chloroform: iso_amyal alcohol --> 25:24:1,   From 5:1 phenol:chloroform?

Thank you
Noor

add 3.8 chloroform and 0.2 isoamyl alcohol. that should give you the proper ratios.

Hmm, I c, Thank you very much,

By the way, I have phenol crystal in the lab, but as I read in internet,  not recommended to use crystal form, Is there any way to prepare stock solution from crystal form?
Many thanks in advance

[mdfenko]

see the cold spring harbor protocols page on phenol.

Edited by mdfenko, 15 December 2010 - 11:33 AM.