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high-throughput retrieval of low volume samples from large well plate


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#1 dtae

dtae

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Posted 11 December 2010 - 08:34 AM

I have 40 500ul 96-well storage plates filled with 20ul DNA samples in each well. The problem I am having is getting a nice high-throughput method to pull 5ul of all of these samples. After spinning down the plate, the liquid tends to pool at the edges of the wells for most wells, but near the bottom of the well for the central wells (the plate is presumably not flat, or bending when it is being spun down). Using an AP96 (96 channel robot) to try to pull 5ul out of this, we consistently have a few missed wells on each plate. Using a 12-channel pippet yields a similar problem--it is hard to line up all of the tips so they retrieve the volume they are supposed to. I don't want to have to use a single channel to pull all of these samples. Any suggestions?

Thanks,
D




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