Posted 10 January 2011 - 06:38 AM
What do you mean by extra? Is it the solution left behind after you pour your gel? Then is your gel ok? If you gel is ok it's not a problem I suppose?
If you gel is not set, first make sure you mix the solution gently to minimize air in your solution (as bob stated the reason), or you may go for de-gasing (Never done this before but if gas is a real problem) . Then check your APS (this is the most likely part that causing un-set gel), prepare a new 10%APS if needed. Once I found my APS powder looked hydrated and my gel didn't set even I prepared APS solution fresh. I borrowed APS powder from other and it worked fine. Then check Temed and Acrylamide.