Electrophoresis: sometimes mA=0
Posted 10 December 2010 - 02:00 AM
I was conducting electrophoresis with TBE 0.5X, and found that the mA reading is 0. I've tried everything like changing the cables, generator and found that when i removed the buffer enough so that the buffer do not cover whole gel, the mA started to increase from 0 to some value. The last time i did electrophoresis, i remember that i cover the whole gel in buffer. What happened? I remember that i read from guideline which says the buffer must completely cover the gel to prevent melting...
I set my mA to be 300 but the max it goes is ~70, usually 30, and sometimes 10. IS this normal? Why can't it reach 300?
Posted 10 December 2010 - 07:22 PM
Posted 11 December 2010 - 04:41 AM
I see. But the buffer in the tank was less than 1 week old... I'll try to replace the buffer next time. Thanks for the advise!
Posted 11 December 2010 - 08:31 PM