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cell biology


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#1 Roopali

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Posted 02 August 2002 - 10:26 AM

What is the best method of acquiring both nuclear and cytoplasmic fractions from transfected cells? I need to check for the expression of a protein both in the nucleus and cytoplasm. Is there a commercial kit available ? Thanks!

#2 yangwenjyh

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Posted 06 August 2002 - 07:45 AM

2ml Dounce cell homogenizer could separate cytosolic and nuclear protein




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