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Antibody gene cloning


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3 replies to this topic

#1 Chelo

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Posted 09 December 2010 - 10:54 AM

Dear forum members,

could anyone tell me how to amplify the gene sequence of an antibody having the mRNA (or cDNA)? What I mean is if there is any consensus sequence in the V-gene and C-gene despite the huge variability of these genes.
Thanks a lot in advance.

#2 BioMiha

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Posted 09 December 2010 - 11:59 AM

You can use degenerated primers or a combination of primers that amplify most encountered sequences. However, these are species dependent - different for mice and humans. I know a good set of primers for mice, humans I have not done before, but I believe it is much the same.

#3 Chelo

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Posted 09 December 2010 - 12:17 PM

You can use degenerated primers or a combination of primers that amplify most encountered sequences. However, these are species dependent - different for mice and humans. I know a good set of primers for mice, humans I have not done before, but I believe it is much the same.

Thanks once more! Could you pass me any reference/paper? I am working with mice antibodies too.

#4 Chelo

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Posted 10 December 2010 - 08:36 AM

Thanks again for the papers!




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