Hi,
I am just started microRNA work, so I don,t know much about this.
I want to know that How we can design real-time pcr primer for microRNA? Or there is any site which contain primer list of known mouse microRNA. I did microRNA array and I got, some microRNA showing different expression with treatment of my compound. so now I want to confirm by Real time.
I know there is some company have commercial Realtime PCR kit, but that one quite expensive for us.
So please if someone can explain about this.
Thank you in anticipating
sincerely
0
Need help
Started by MicroRNAQa, Dec 08 2010 11:47 PM
1 reply to this topic
#2
UBClabbie
-
- Active Members
-
- 65 posts
Enthusiast
5
Neutral
Neutral
Posted 19 January 2011 - 03:13 PM
What company were you looking at for real-time analysis?
You can order individual primer sets for miRNA here: http://www.abmgood.c...11839&dsn=12258
Its relatively inexpensive. There's also a RT-qPCR profiling service that would also help to confirm your profiling. Microarray is not really that reliable.
You can order individual primer sets for miRNA here: http://www.abmgood.c...11839&dsn=12258
Its relatively inexpensive. There's also a RT-qPCR profiling service that would also help to confirm your profiling. Microarray is not really that reliable.
