Nuclear extraction on non-primary culture cells
Posted 08 December 2010 - 06:50 AM
Would anyone know how to extract nuclear proteins from neural stem cells and/or peripheral blood mononuclear cells?
I've been having trouble getting a signal for this protein of interest, and I've tried troubleshooting everything from harvesting whole-cell lysate to western blotting (e.g. increase protein concentration loaded into each well, increase antibody concentration, change buffer concentrations, wash more/less, etc).
Or if someone can recommend a good company, that'd be a great starting point too. I'm actually looking into Millipore's protocol, but still wanted more suggestions/advice from the general crowd.
Posted 08 December 2010 - 07:41 AM
Posted 09 December 2010 - 09:24 AM
Thanks! I checked out the paper. Going to keep you posted if this works. It's my first attempt....