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primer and promoter sequences


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#1 seed

seed

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Posted 08 December 2010 - 04:52 AM

I'm new plant transformation and now I have to isolate RNA(ARR22 gene, At3g04280) from the Arabidopsis. I'm going to do rt-pcr where I have to find the sequence for the promoter and the primer. Please anyone guide me on how to get the sequence. I already try it using primer3 and PlantCare(promoter sequence), but I'm not sure whether they are the right sequences or not. Besides, the sequences that I have are difference from the previous study. I also have to find the sequence for tomato plant (SL1.00sc06004_225.1.1)(homologues with Arabidopsis ARR22 gene).

#2 criscastells

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Posted 13 December 2010 - 03:06 AM

Try ensembl DB. It worked fine for me :P
Hope to be helpful




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