Standard curve for real-time PCR
Posted 08 December 2010 - 02:12 AM
I am trying to make standard curve for real-time PCR. I have amplified the cDNA using gene specific primers and then run it on gel. Now I am about to cut the specific fragment in gel, purify and quantify it. It seems very complicated and sensitive.
Can I use the cDNA (got by using OligoDT) for making standard curve dilution for real-time PCR?
Posted 08 December 2010 - 09:32 AM
Posted 09 December 2010 - 03:02 AM