I have an around 4k bps DNA fragment. It comes from restriction digestion. I want to fragment it in several small pieces and clone them into an vector and then do sequencing. This 4k sequence data is unknown, and it is failed with PCR amplification. I have 2 questions regarding the experiment. The first one is which strategy I can use for cut the 4k DNA into a couple of small fragments, the best choice is 500 bps for each fragment. The second question is that which cloning kit I can use to cloning these small fragments.
Any recommendation will be highly appreciated.
Edited by subtelo, 07 December 2010 - 09:38 AM.