2 replies to this topic

[BioMiha]

Hi all,

I am wondering which subset of B cells is used to make hybridomas. Or, alternatively, which marker would differentiate them. Are they plasmablasts, plasma cells or memory B cells. I can't find this information anywhere, yet I would expect this to be common knowledge by now.
Best regards,
Miha

[Chelo]

BioMiha, on 07 December 2010 - 06:45 AM, said:

Hi all,

I am wondering which subset of B cells is used to make hybridomas. Or, alternatively, which marker would differentiate them. Are they plasmablasts, plasma cells or memory B cells. I can't find this information anywhere, yet I would expect this to be common knowledge by now.
Best regards,
Miha

Theoretically, any B-cell can fuse to produce hybridomas. T-cells too!

[BioMiha]

That may be true, however the splenocytes that are used for fusion are activated B cells, that have undergone affinity maturation and class switching. The B cells that have undergone affinity maturation are germinal center B cells that are either plasmablasts, fully differentiated plasma cells or memory B cells.