Is it normal for fetal calf serum to be cloudy (precipitated) ?
Are they normaly more cloudy than FBS?
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#2
Rumpel
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Posted 07 December 2010 - 07:46 AM
Hi!
FCS and FBS are the same.
Our experience is that if you store FCS at -20°C or -80°C and unfreeze it slowly you'll probably have precipitates. It's better to unfreeze it at room temperature and to invert it once in a while.
FCS and FBS are the same.
Our experience is that if you store FCS at -20°C or -80°C and unfreeze it slowly you'll probably have precipitates. It's better to unfreeze it at room temperature and to invert it once in a while.
#3
Alvolo
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Posted 11 December 2010 - 06:45 AM
Rumpel, on 07 December 2010 - 07:46 AM, said:
Hi!
FCS and FBS are the same.
Our experience is that if you store FCS at -20°C or -80°C and unfreeze it slowly you'll probably have precipitates. It's better to unfreeze it at room temperature and to invert it once in a while.
FCS and FBS are the same.
Our experience is that if you store FCS at -20°C or -80°C and unfreeze it slowly you'll probably have precipitates. It's better to unfreeze it at room temperature and to invert it once in a while.
thank you,but may i know what do you mean by unfreeze slowly?
water bath with 37 celcius consider slow or fast?
#4
Rumpel
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Posted 11 December 2010 - 12:45 PM
Alvolo, on 11 December 2010 - 06:45 AM, said:
Rumpel, on 07 December 2010 - 07:46 AM, said:
Hi!
FCS and FBS are the same.
Our experience is that if you store FCS at -20°C or -80°C and unfreeze it slowly you'll probably have precipitates. It's better to unfreeze it at room temperature and to invert it once in a while.
FCS and FBS are the same.
Our experience is that if you store FCS at -20°C or -80°C and unfreeze it slowly you'll probably have precipitates. It's better to unfreeze it at room temperature and to invert it once in a while.
thank you,but may i know what do you mean by unfreeze slowly?
water bath with 37 celcius consider slow or fast?
37°C would be pretty fast, room temperature is fast enough, usually.
#5
To dream to do
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Posted 01 February 2011 - 12:30 AM
Rumpel, on 11 December 2010 - 12:45 PM, said:
Alvolo, on 11 December 2010 - 06:45 AM, said:
Rumpel, on 07 December 2010 - 07:46 AM, said:
Hi!
FCS and FBS are the same.
Our experience is that if you store FCS at -20°C or -80°C and unfreeze it slowly you'll probably have precipitates. It's better to unfreeze it at room temperature and to invert it once in a while.
FCS and FBS are the same.
Our experience is that if you store FCS at -20°C or -80°C and unfreeze it slowly you'll probably have precipitates. It's better to unfreeze it at room temperature and to invert it once in a while.
thank you,but may i know what do you mean by unfreeze slowly?
water bath with 37 celcius consider slow or fast?
37°C would be pretty fast, room temperature is fast enough, usually.
It is frozen at -20C and I keep it at 4c(in refrigerator)overnight before using in next day.
#6
lab rat
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Posted 03 February 2011 - 08:08 PM
In my old lab, we would put the frozen FBS in the same 37C water bath as the medium. Doesn't make a difference, really.
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.
