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Fetal calf serum


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#1 Alvolo

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Posted 07 December 2010 - 06:36 AM

Is it normal for fetal calf serum to be cloudy (precipitated) ?
Are they normaly more cloudy than FBS?

#2 Rumpel

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Posted 07 December 2010 - 07:46 AM

Hi!

FCS and FBS are the same.
Our experience is that if you store FCS at -20C or -80C and unfreeze it slowly you'll probably have precipitates. It's better to unfreeze it at room temperature and to invert it once in a while.

#3 Alvolo

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Posted 11 December 2010 - 06:45 AM

Hi!

FCS and FBS are the same.
Our experience is that if you store FCS at -20C or -80C and unfreeze it slowly you'll probably have precipitates. It's better to unfreeze it at room temperature and to invert it once in a while.


thank you,but may i know what do you mean by unfreeze slowly?
water bath with 37 celcius consider slow or fast?

#4 Rumpel

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Posted 11 December 2010 - 12:45 PM


Hi!

FCS and FBS are the same.
Our experience is that if you store FCS at -20C or -80C and unfreeze it slowly you'll probably have precipitates. It's better to unfreeze it at room temperature and to invert it once in a while.


thank you,but may i know what do you mean by unfreeze slowly?
water bath with 37 celcius consider slow or fast?


37C would be pretty fast, room temperature is fast enough, usually.

#5 To dream to do

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Posted 01 February 2011 - 12:30 AM



Hi!

FCS and FBS are the same.
Our experience is that if you store FCS at -20C or -80C and unfreeze it slowly you'll probably have precipitates. It's better to unfreeze it at room temperature and to invert it once in a while.


thank you,but may i know what do you mean by unfreeze slowly?
water bath with 37 celcius consider slow or fast?


37C would be pretty fast, room temperature is fast enough, usually.


It is frozen at -20C and I keep it at 4c(in refrigerator)overnight before using in next day.

#6 lab rat

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Posted 03 February 2011 - 08:08 PM

In my old lab, we would put the frozen FBS in the same 37C water bath as the medium. Doesn't make a difference, really.
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.




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