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designing of primers


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#1 deespike

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Posted 07 December 2010 - 05:25 AM

Hi all

I am designing primers for pcr amplification of 2.3kb insert and I need to add salI primers at the 5' end of both the primers.Can anyone please suggest about the appropriate bases that I need to incorporate before the restriction site at the 5' end?


Thanks in advance

#2 Rsm

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Posted 07 December 2010 - 06:37 AM

ttt
I got soul, but I'm not a soldier

#3 seed

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Posted 07 December 2010 - 03:35 PM

I'm new with plant transformation and rt-pcr. Please help me find promoter and primer sequences for Arabidopsis and tomato plant. And please let me know, if there are any factors that I have to consider in order to do rt-pcr.




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