Propagating ccdB survival cells
Posted 06 December 2010 - 11:20 AM
- dnatrash likes this
Posted 06 December 2010 - 12:45 PM
Posted 06 December 2010 - 01:12 PM
Posted 06 December 2010 - 06:26 PM
Posted 24 January 2012 - 02:09 AM
I am thinking of preparing ccdB survival competent cells for our lab use. I read your discussion in the forum. Wonder, how did it go? How much was the efficiency you could achieve?
Thank you in advance.
Posted 24 January 2012 - 06:00 AM
Posted 17 August 2013 - 02:42 PM
Following you guys' discussion,
I ran out invitrogen DB3.1 stock, and want to make my own ccdb competent cells. Tried once, but efficiency for ccdb vector is extremely low despite high competency for pUC19.
Wonder whether I should add streptomycin to maintain genotype/ccdb resistance of DB3.1 due to its StrpR genotype.
Are there any other tricks to maintain ccdb resistance?
Thanks so much for advice,