What might be the problem if my northern blots show very weak signals or have lots of smeary stuff?
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bob1
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Thelymitra pulchella
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Excellent
Excellent
Posted 05 December 2010 - 02:15 PM
transfer problems, running conditions, RNase presence, low radio-nucleide incorporation (or DIG if you are using it), not enough substrate (DIG), annealing temp too high or too low... etc,
Pretty much anything. Can you post your protocol?
Pretty much anything. Can you post your protocol?
