Western difficulty in primary neuron cells.
Posted 04 December 2010 - 10:07 AM
I cultured primary neurons on 6-well plates and use 150ul lysis buffer per well (My guess is that neurons are much less dense than HEK293 cells so that the protein levels would be lower in lysate), western is done with Cell Signaling antibodies, Li-Cor 2nd antibodies and imaging system.
Posted 20 February 2013 - 02:40 PM
I always had an alternate hypothesis....