Interpretation of Ct values
Posted 03 December 2010 - 11:57 PM
I am studying the expression of phosphate regulatory genes of Rhizobium using Real time PCR. I performed the Real time PCR using SYBR green kit and ABI 7500 PCR system. After normalization of the data, I got these Ct values
1. Endogenous control (16S rRNA) Ct: 13.25
2. Gene 1: phoB: 12.42
3. Gene 2 : phoC: 12.11
4. Gene 3: phoA: 14.64
I learnt from the Real time PCR manual that the Ct value is a measure of the abundance of the gene/ mRNA. Lower Ct values mean high abundance and higher Ct means low abundance
Does this mean that genes 1,2 are expressed at a higher level than that of the endogenous control?
and gene 3 is expressed at a lower level than the endogenous control?
Posted 06 December 2010 - 01:05 AM
Posted 08 December 2010 - 09:41 AM