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ELISA results: pg/ml


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#1 ddm

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Posted 03 December 2010 - 05:10 PM

We have performed ELISA's on tissue. We reported data as pg/ml. Total protein determination was not done and tissue homogenates were discarded. Question: Can data be repored as pg/ml? Is there a way to normalize the ELISA data?
Any advice is welcome.
ddm

#2 sgt4boston

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Posted 07 December 2010 - 06:48 AM

You must have a very sensitive assay! Great! Results can be reported in mg/ml, ug/ml ng/ml pg/ml...

To normalize data...you will need some common points analyzed by the same method that was done at 2 different times.

#3 ujla80

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Posted 17 December 2010 - 10:48 PM

in case of serum sample also we report data as pg/ml...then why not homogenate...i have come across a paper where elisa was done using mouse lung homogenate and cytokine levels are expressed as pg/ml..i think u should not worry and go ahead.





We have performed ELISA's on tissue. We reported data as pg/ml. Total protein determination was not done and tissue homogenates were discarded. Question: Can data be repored as pg/ml? Is there a way to normalize the ELISA data?
Any advice is welcome.
ddm


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