We have performed ELISA's on tissue. We reported data as pg/ml. Total protein determination was not done and tissue homogenates were discarded. Question: Can data be repored as pg/ml? Is there a way to normalize the ELISA data?
Any advice is welcome.
ddm
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#2
sgt4boston
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Posted 07 December 2010 - 06:48 AM
You must have a very sensitive assay! Great! Results can be reported in mg/ml, ug/ml ng/ml pg/ml...
To normalize data...you will need some common points analyzed by the same method that was done at 2 different times.
To normalize data...you will need some common points analyzed by the same method that was done at 2 different times.
#3
ujla80
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Posted 17 December 2010 - 10:48 PM
in case of serum sample also we report data as pg/ml...then why not homogenate...i have come across a paper where elisa was done using mouse lung homogenate and cytokine levels are expressed as pg/ml..i think u should not worry and go ahead.
ddm, on 03 December 2010 - 05:10 PM, said:
We have performed ELISA's on tissue. We reported data as pg/ml. Total protein determination was not done and tissue homogenates were discarded. Question: Can data be repored as pg/ml? Is there a way to normalize the ELISA data?
Any advice is welcome.
ddm
Any advice is welcome.
ddm
god is great
