I would like to differentiate CD11b monocytes (isolated from mouse) into macrophages. Some papers described that treatment for 24h with 20nM of PMA of THP-1 cells will induce a macrophage phenotype. Does it work also for CD11b monocytes or is there a better/standard treatment?
Thanks a lot
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Differentiation of CD11b cells into macrophages.Help please
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