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Differentiation of CD11b cells into macrophages.Help please


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#1 Sbdt

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Posted 03 December 2010 - 01:29 PM

Hi,

I would like to differentiate CD11b monocytes (isolated from mouse) into macrophages. Some papers described that treatment for 24h with 20nM of PMA of THP-1 cells will induce a macrophage phenotype. Does it work also for CD11b monocytes or is there a better/standard treatment?

Thanks a lot

#2 Piersgb

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Posted 13 December 2010 - 04:15 AM

GM-CSF and M-CSFare the two growth factors giving rise to monocyte/macrophage-restricted progeny.

http://nic.sav.sk/lo...fic/node21.html




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