Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Question about RT...


  • Please log in to reply
1 reply to this topic

#1 Jade

Jade

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 03 December 2010 - 10:11 AM

Hey I'm new to this so here goes:

My mentor told me that in RT the cDNA produced by the reaction has the same weight as the RNA used in the reaction. How does that work out?

Thanks for your replies!

Jade

#2 WSN

WSN

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 39 posts
0
Neutral

Posted 10 December 2010 - 04:49 PM

Maybe more appropriately it's the molar amount that would be same, given that the conversion efficiency is 100 percent. Same molar amount of cDNA would for sure be lighter than the starting RNA, because it's DEOXY-.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.