I've been performing in situ hybridisation on crysections. The colour comes up well, however there seems to be a lot of specks of something on the slides which werent there to begin with. So my slides look dirty and are difficult to photograph. The post-doc in the lab has been having the same problem.
I make up fresh solutions everytime and also keep my reaction mix fresh..
Any ideas as to what could be causing this?? I heard something about MgCl2 in the NTMT being an issue?
A bit lost
Submit your paper to J Biol Methods today!
ISH on Cryosections
No replies to this topic