Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

ISH on Cryosections

  • Please log in to reply
No replies to this topic

#1 Htastic77



  • Members
  • Pip
  • 2 posts

Posted 03 December 2010 - 02:58 AM

Hi folks

I've been performing in situ hybridisation on crysections. The colour comes up well, however there seems to be a lot of specks of something on the slides which werent there to begin with. So my slides look dirty and are difficult to photograph. The post-doc in the lab has been having the same problem.

I make up fresh solutions everytime and also keep my reaction mix fresh..

Any ideas as to what could be causing this?? I heard something about MgCl2 in the NTMT being an issue?

A bit lost :blink:

H x

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.