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ISH on Cryosections


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#1 Htastic77

Htastic77

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Posted 03 December 2010 - 02:58 AM

Hi folks

I've been performing in situ hybridisation on crysections. The colour comes up well, however there seems to be a lot of specks of something on the slides which werent there to begin with. So my slides look dirty and are difficult to photograph. The post-doc in the lab has been having the same problem.

I make up fresh solutions everytime and also keep my reaction mix fresh..

Any ideas as to what could be causing this?? I heard something about MgCl2 in the NTMT being an issue?

A bit lost :blink:

H x





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