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single gene amplification


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#1 aashu

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Posted 03 December 2010 - 01:14 AM

I'm trying to amplify the protein kinase gene involved in drought tolerence; i've checked my rna with gel, and I also amplified cDNA. The PCR product is showing high amount of primer dimers and the band is not very much distinct. still i have eluted and reamplified that band but still I AM GETTING PRIMER DIMER AND VERY LESS CONCENTRATION. so can be the reason whether my primer desigfning ok any other. please anybody reply....
Thanks anyway in advance

#2 mdfenko

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Posted 03 December 2010 - 07:53 AM

you may want to redesign your primers and check them for dimer formation before ordering them.
talent does what it can
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#3 aashu

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Posted 03 December 2010 - 10:24 PM

you may want to redesign your primers and check them for dimer formation before ordering them.

I got a good band and less primer dimer after diluting primer. I think my primer works well. Anyway thank you for sugestion.




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