I'm trying to amplify the protein kinase gene involved in drought tolerence; i've checked my rna with gel, and I also amplified cDNA. The PCR product is showing high amount of primer dimers and the band is not very much distinct. still i have eluted and reamplified that band but still I AM GETTING PRIMER DIMER AND VERY LESS CONCENTRATION. so can be the reason whether my primer desigfning ok any other. please anybody reply....
Thanks anyway in advance
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single gene amplification
Started by aashu, Dec 03 2010 01:14 AM
2 replies to this topic
#2
mdfenko
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Posted 03 December 2010 - 07:53 AM
you may want to redesign your primers and check them for dimer formation before ordering them.
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#3
aashu
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Posted 03 December 2010 - 10:24 PM
mdfenko, on 03 December 2010 - 07:53 AM, said:
you may want to redesign your primers and check them for dimer formation before ordering them.
