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ChIP-chip input controls and related analysis


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#1 boji

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Posted 02 December 2010 - 09:24 PM

Is there a difference in the way ChIP-chip data should be analyzed depending on if the "input" is the conventional genomic DNA versus a mock-ChIP'ed sample (ie. with an isotype control antibody)??? The values (ie. log2(Test/input) that we get seem to be much lower when using a mock-ChIP'ed input versus a conventional total input.

Thanks!

#2 honey

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Posted 18 December 2010 - 03:26 AM

Is there a difference in the way ChIP-chip data should be analyzed depending on if the "input" is the conventional genomic DNA versus a mock-ChIP'ed sample (ie. with an isotype control antibody)??? The values (ie. log2(Test/input) that we get seem to be much lower when using a mock-ChIP'ed input versus a conventional total input.

Thanks!


Chip input should be ideal




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