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Designing primer to remove his-tag


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#1 xiaolongbao

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Posted 02 December 2010 - 11:47 AM

Inorganic chemist trying to do biochemistry here....

I have a protein that is his-tagged at both the N- and C-terms. I want to get rid of the his-tags and just amplify the gene of the wild-type. There is no type of cleavage site after the his-tag. How would I design a primer that removes the his-tag from either end? Should I use an internal primer?
Thanks!

#2 Adrian K

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Posted 06 December 2010 - 10:12 AM

I would suggest you buy a new expression vector, re-clone the gene again with stop codon.
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