Inorganic chemist trying to do biochemistry here....
I have a protein that is his-tagged at both the N- and C-terms. I want to get rid of the his-tags and just amplify the gene of the wild-type. There is no type of cleavage site after the his-tag. How would I design a primer that removes the his-tag from either end? Should I use an internal primer?
Thanks!
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Adrian K
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Posted 06 December 2010 - 10:12 AM
I would suggest you buy a new expression vector, re-clone the gene again with stop codon.
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..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
