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Homogenization for Plasma Membrane Protein Extraction


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#1 cancerbio

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Posted 02 December 2010 - 08:46 AM

Hi!

I am using the BioVision plasma membrane protein extraction kit (Cat K268-50) which says to use a dounce homogenizer for lysing cells. Would it be possible to instead use a mechanical homogenizer at low speeds for purposes of extracting only plasma membrane proteins?

Thanks!

#2 mdfenko

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Posted 03 December 2010 - 09:15 AM

using a dounce is more about pestle clearance (how tight the fit is) than other homogenizers. you can be gentler with a dounce.
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