Ok, I have a question involving making a primer working stock by combining the F+R primer
So, I have my F and R primers seperately at 100micromolar. I want to make 200microliters of a 7.5micromolar working stock. When I set up C1V1=C2V2, I get: V1= [(7.5uM)(200ul)]/(100uM)= 15ul. However, does this 15ul actually mean 30ul since the F and R are seperately at 100uM? To perhaps clarify better, am I adding 7.5ul of the F and 7.5 of the R to 185ul of H20? Or, am I adding 15ul of Forward + 15 ul of Reverse to 170ul H20?
I would greatly appreciate any help.
PCR Primer Dilution from F+R
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