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[ascaris]

Hi All ,
I want to stain helminth eggs but the problem is it has a lipid layer which is highly impermeable. Does any one have any idea about improving the permeability of lipid layer ( espeacially ascaroside layer)? like treating with xylene, or sonication or electoporation?

[genehunter]

Cold Aceton treatment should fix the cells and dissolve away lipids along the way.