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lipid pemeability


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#1 ascaris

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Posted 02 December 2010 - 06:24 AM

Hi All ,
I want to stain helminth eggs but the problem is it has a lipid layer which is highly impermeable. Does any one have any idea about improving the permeability of lipid layer ( espeacially ascaroside layer)? like treating with xylene, or sonication or electoporation?

#2 genehunter

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Posted 13 December 2010 - 08:51 AM

Cold Aceton treatment should fix the cells and dissolve away lipids along the way.




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