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lipid permeability


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#1 ascaris

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Posted 02 December 2010 - 06:21 AM

Hi All ,
I want to stain helminth eggs but the problem is it has a lipid layer which is highly impermeable. Does any one have any idea about improving the permeability of lipid layer ( espeacially ascaroside layer)? like treating with xylene, or sonication or electoporation?

Edited by ascaris, 02 December 2010 - 06:21 AM.


#2 bob1

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Posted 02 December 2010 - 02:02 PM

What have you tried so far? I would start with detergents - SDS, triton X-100, NP-40, etc.

#3 ascaris

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Posted 03 December 2010 - 07:13 AM

What have you tried so far? I would start with detergents - SDS, triton X-100, NP-40, etc.

Thankyou very much for the reply. I have not yet tried any but planning to pretreat the eggs with some enzymes.But will try some of the detergents you mentioned.




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