2 replies to this topic

[ascaris]

Hi All ,
I want to stain helminth eggs but the problem is it has a lipid layer which is highly impermeable. Does any one have any idea about improving the permeability of lipid layer ( espeacially ascaroside layer)? like treating with xylene, or sonication or electoporation?

Edited by ascaris, 02 December 2010 - 06:21 AM.

[bob1]

What have you tried so far?  I would start with detergents - SDS, triton X-100, NP-40, etc.

[ascaris]

bob1, on 02 December 2010 - 02:02 PM, said:

What have you tried so far?  I would start with detergents - SDS, triton X-100, NP-40, etc.

Thankyou very much for the reply. I have not yet tried any but planning to pretreat the eggs with some enzymes.But will try some of the detergents you mentioned.