LB broth very dark colour
Posted 02 December 2010 - 04:57 AM
Posted 02 December 2010 - 05:59 AM
What this means is that there is less than usual amount of sugar and amino acids in the media. And higher than usual concentration of millard reaction products in the media. Millard reaction products are growth inhibitory to most bacteria. However E. coli has some tolerates to millard reaction product.
Now the question is can the media still be used?
It depends... how brown is brown here? If it is on the side of dark brown going to black... the media can't be used. If the media is still golden in colour, it is okay. A picture would be helpful.
Posted 02 December 2010 - 08:37 AM
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that did belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
Posted 03 December 2010 - 12:47 AM
Posted 03 December 2010 - 03:57 AM
Did you reverse the quantity of yeast extract and tryptone or just typed wrongly? My recipe is 10g tryptone and 5g YE per litre LB.
Erm I think you might be right. oops. I have just stuck another bottle in the autoclave using the same recipe as well, assuming it was due to the bottle or someone autoclaved it twice or something.
Oh well that clears that up, I should copy down my recipes more carefully next time...
Posted 06 December 2010 - 07:18 AM
can I just check I am getting this right?
5g Yeast Extract
1L MilliQ water
mix, then autoclave with loose lid
because I seriously cannot work out what I am doing wrong anymore