Can someone inform a non-chemist what purpose it serves in the DNA extraction of ChIPed material? Is it necessary? And what informality could I be missing if I do not use incorporate it into my DNA extractions to check for fragmentation quality? Thanks in advance to all who can help with these inquiries.
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#1
chabraha
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Posted 01 December 2010 - 11:51 AM
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#2
KPDE
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Posted 01 December 2010 - 05:55 PM
As far as I know it is an anti-foaming agent and an agent that makes the organic/aqueous interface more defined.
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perneseblue
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Posted 02 December 2010 - 06:11 AM
KPDE, on 01 December 2010 - 05:55 PM, said:
As far as I know it is an anti-foaming agent and an agent that makes the organic/aqueous interface more defined.
in reference to phenol-chloroform extraction
May your PCR products be long, your protocols short and your boss on holiday
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chabraha
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Posted 06 December 2010 - 10:07 AM
cool, definitely not what I thought it was for. For some reason I was under the impression that it helped extract either small DNA fragments or gDNA, although I have no idea from where I acquired these impressions. Thanks for the help KPDE.
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