Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

primer reconstitution


  • Please log in to reply
4 replies to this topic

#1 miss-pcr

miss-pcr

    member

  • Active Members
  • Pip
  • 11 posts
0
Neutral

Posted 01 December 2010 - 04:06 AM

Hi...
I would like to ask why sometimes we reconstitute primers in water or TE buffer...Thanks

#2 BioMiha

BioMiha

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 225 posts
8
Neutral

Posted 01 December 2010 - 04:38 AM

The primers are stable for a longer period of time in TE buffer, however the EDTA in TE can inhibit downstream reactions, that is why for PCR primers should be either diluted or stored in water.

#3 ivanbio

ivanbio

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 116 posts
7
Neutral

Posted 01 December 2010 - 09:55 AM

It is a good idea to reconstitute your primers in TE when preparing permanent stocks (100 uM) since they are more stable that way. By the time you dilute them for PCR (1 uM final concentration) the EDTA is too dilute to inhibit PCR.

Ivan
Carlsbad, CA

#4 miss-pcr

miss-pcr

    member

  • Active Members
  • Pip
  • 11 posts
0
Neutral

Posted 02 December 2010 - 03:08 AM

The primers are stable for a longer period of time in TE buffer, however the EDTA in TE can inhibit downstream reactions, that is why for PCR primers should be either diluted or stored in water.

Thank you very much for your reply and cooperation :)

#5 miss-pcr

miss-pcr

    member

  • Active Members
  • Pip
  • 11 posts
0
Neutral

Posted 02 December 2010 - 03:10 AM

It is a good idea to reconstitute your primers in TE when preparing permanent stocks (100 uM) since they are more stable that way. By the time you dilute them for PCR (1 uM final concentration) the EDTA is too dilute to inhibit PCR.

Thank you very much for your reply and cooperation :)




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.