4 replies to this topic

[miss-pcr]

Hi...
I would like to ask why sometimes we reconstitute primers in water or TE buffer...Thanks

[BioMiha]

The primers are stable for a longer period of time in TE buffer, however the EDTA in TE can inhibit downstream reactions, that is why for PCR primers should be either diluted or stored in water.

[ivanbio]

It is a good idea to reconstitute your primers in TE when preparing permanent stocks (100 uM) since they are more stable that way. By the time you dilute them for PCR (1 uM final concentration) the EDTA is too dilute to inhibit PCR.

[miss-pcr]

BioMiha, on 01 December 2010 - 04:38 AM, said:

The primers are stable for a longer period of time in TE buffer, however the EDTA in TE can inhibit downstream reactions, that is why for PCR primers should be either diluted or stored in water.

Thank you very much for your reply and cooperation

[miss-pcr]

ivanbio, on 01 December 2010 - 09:55 AM, said:

It is a good idea to reconstitute your primers in TE when preparing permanent stocks (100 uM) since they are more stable that way. By the time you dilute them for PCR (1 uM final concentration) the EDTA is too dilute to inhibit PCR.

Thank you very much for your reply and cooperation