Posted 01 December 2010 - 01:58 AM
An unspecific band appear at 55kDa on my western blot, i suppose it is IgG.
my protein of interest is at 50kDa!
How can i get rid of IgG band?
Posted 01 December 2010 - 08:30 AM
genius does what it must
i do what i get paid to do
Posted 01 December 2010 - 12:06 PM
Otherwise, you can use protein A-HRP as your secondary. It isn't as sensitive as most other secondaries but it will only bind antibodies in the native conformation. This means that it can't bind the denatured heavy or light chain that was used in the IP and run on the gel.