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Positive Product in semi-quantitative RT-PCR


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#1 Banana

Banana

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Posted 30 November 2010 - 09:02 AM

"What would you expect to see as a positive product when the target mRNA expression is examined using semi-quantitative RT-PCR?"

Hi. I am a student and this question has come up with regards to one of my assignments. I am pretty sure that the RT-PCR in this case denotes reverse transcription PCR rather than real-time.

Is this a trick question, or is the answer simply: If the mRNA is expressed in the examined tissue, a bright band will form due to the amplification of the DNA sample by the specifically designed primers that anneal downstream and upstream to the coding region of the gene.

Thanks in advance for any help... I have never asked for any help on forums before, but I was just a bit confused with this one.




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