Effect of DTT on the affinity of Protein A/G and immune complex
Posted 29 November 2010 - 06:26 PM
We are performing a proteomic approach on the ICs, my colleagues suggest an "on beads" treatment of DTT and Iodoacetamidd for immune complex before elution step.
Do you have any idea how these DTT and Iodoacetamidd affect on the binding of immune complex and protein A/G?
Posted 29 November 2010 - 11:23 PM
Posted 30 November 2010 - 01:42 AM