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red ponceau


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3 replies to this topic

#1 jasmina

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Posted 29 November 2010 - 02:43 PM

hi,
do u know the ingredients of ponceau staining, efficient to check the transfert on PVDF filter??
thanks

#2 mdfenko

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Posted 30 November 2010 - 12:09 PM

sigma sells a full strength solution that is 0.1% ponceau s in 5% acetic acid.

Edited by mdfenko, 30 November 2010 - 12:09 PM.

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#3 jasmina

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Posted 02 February 2011 - 01:33 PM

sigma sells a full strength solution that is 0.1% ponceau s in 5% acetic acid.

I prepared ponceau as the ingredient you suggested, i couldnt see the lanes!! but the marker is well transferred.
so, i dont understand what is the problem, is it the ponceau (it looks not concentrated). or PVDF filter,or..

#4 mdfenko

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Posted 04 February 2011 - 10:49 AM

are you sure the ponceau was completely (or nearly) solubilized? the solution may have been weak.

if it is weak then you could stain for a longer time.
talent does what it can
genius does what it must
i do what i get paid to do




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