Help Me With Polyclonal Production
Posted 29 November 2010 - 12:16 PM
What we did:
We purified rabbit serum and ran an SDS page and notices that some of purifications has extra third band around 80 kda (not from the light and heavy chains). Can someone please help to explain why this is happening?
Could it be because of the way we are bleeding the rabbits or the way we are collecting the serum? What is the best way to do this? Has anyone else had an extra band in their serum?
Posted 29 November 2010 - 01:33 PM
Posted 29 November 2010 - 01:44 PM
Posted 29 November 2010 - 10:37 PM
in all the sera, and has a MW in the range that you say. Good luck
Posted 10 December 2010 - 12:16 AM
Another possibility is IgM, it has a molecular weight around that value.
Please explain how you are 'purify' your antibody.
Posted 10 December 2010 - 03:20 AM