how the seperate dead cells from living ones.
Posted 03 August 2002 - 04:41 AM
Posted 05 August 2002 - 09:56 AM
I would think if you have the cells already in culture you just tripsinate them and transfer them to a new plate. Dead cells don't reattach so you can rinse the plate after a day or so and get rid of the dead ones.
Posted 06 August 2002 - 01:58 AM
(Edited by brian at 3:00 am on Aug. 6, 2001)
Posted 06 August 2002 - 05:00 AM
An alternative would be to use propidium iodide. This stains necrotic cells. After staining the cells can be sorted by FACS. I don't have the exact protocol because I haven't done it myself, but it seems to be a widely used method.
Ficol may be used to separate the cells. I only know it works for lyphocytes. I'm not certain which other celltypes might be included.
Posted 26 December 2004 - 12:04 AM