Did you use Invivofectamine (Invitrogen) ?

Started by Berga, Nov 29 2010 05:01 AM

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#1 Berga

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Posted 29 November 2010 - 05:01 AM

Hi people!

I will start up a set of experiments in which we want to knock down some genes in the mouse liver specifically. A few days ago, I got an email from a colleague who thought that the invivofectamine from Invitrogen could be worth checking out. I have no previous experience with knocking down genes and therefore I want to ask the users of this system:

- Did it work well?

- Anything you should watch out for?

- Pros and cons, anything you noticed..? =)

Thanks in advance
/Berga

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#2 genehunter

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Posted 13 December 2010 - 08:44 PM

Try hydrodynamic injection!!!

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#3 96well

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Posted 14 December 2010 - 05:50 AM

I quote genehunter!

Nature magazine. Do you qualify for a free subscription?

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#4 Xavier2

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Posted 03 January 2011 - 12:29 PM

I used Invivofectamine2.0 to knock down gene specifically in mouse liver, it worked awesome !! Great knock down and very long effect more than 2 weeks at 5mg/kg and no need to do hydrodynamic injection.

I totally recommend it ! good luck,

Xavier

Berga, on 29 November 2010 - 05:01 AM, said:

Hi people!

I will start up a set of experiments in which we want to knock down some genes in the mouse liver specifically. A few days ago, I got an email from a colleague who thought that the invivofectamine from Invitrogen could be worth checking out. I have no previous experience with knocking down genes and therefore I want to ask the users of this system:

- Did it work well?

- Anything you should watch out for?

- Pros and cons, anything you noticed..? =)

Thanks in advance
/Berga

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