Posted 26 November 2010 - 02:04 PM
I'm working in a lab which houses two different groups and they both have slightly different protocols for RNA extraction and purification. For the washing step, our protocol says to use 75% ethanol whilst the other group says to use 85%. I asked the technician and he said that he once read a paper that suggested 85% was better, and I'm just wondering why. Does it help to prevent the RNA from dissolving? Would this be at the expense of salt-removal? Their protocol only says to wash a single time.