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antigen purification

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#1 anonymous



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Posted 01 June 2001 - 09:00 PM

Hi,I would like to identify an antigen recognized by an antibody. I was thinking about making a column with the antibody, use cell lysates from the cell linethat has been shown to have high expression of the antigen, then run a 2d-geland sequence the protein.Any suggestions on protocols on how to make the column, treat the cell lysatesand other comments?Thanks, Menno de Winther

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