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How to Isolate T-cell from human PBMC?

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#1 aliceng



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Posted 24 November 2010 - 06:38 PM

I want to isolate T-cells from human PBMC for the study of immunosuppressive effect of drug, and also the proliferation of T-cells. I have used nylon wool column to isolate T-cell from human PBMC, the human T-cells only agglutinated but XTT assay showed no proliferation after 72hr PHA/ConA treatment. Now, I want to buy a kit for T-cell isolation, any idea?

#2 Denis Baev

Denis Baev


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Posted 19 January 2011 - 08:28 AM

The best way to isolate T cells untouched from PBMCs (or from whole blood) is to use negative-T cell selection MACS kit from Miltenyi Biotec

Edited by Denis Baev, 19 January 2011 - 08:31 AM.

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