Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How to Isolate T-cell from human PBMC?


  • Please log in to reply
1 reply to this topic

#1 aliceng

aliceng

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 24 November 2010 - 06:38 PM

I want to isolate T-cells from human PBMC for the study of immunosuppressive effect of drug, and also the proliferation of T-cells. I have used nylon wool column to isolate T-cell from human PBMC, the human T-cells only agglutinated but XTT assay showed no proliferation after 72hr PHA/ConA treatment. Now, I want to buy a kit for T-cell isolation, any idea?

#2 Denis Baev

Denis Baev

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 53 posts
1
Neutral

Posted 19 January 2011 - 08:28 AM

The best way to isolate T cells untouched from PBMCs (or from whole blood) is to use negative-T cell selection MACS kit from Miltenyi Biotec

Edited by Denis Baev, 19 January 2011 - 08:31 AM.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.