Can the MTS assay for control and treatment group carry out separately at diffe
Posted 24 November 2010 - 03:45 AM
However, i am now working as a RA and we are required to measure proliferation rate of cancer cell. Some experienced colleagues told me that there is not much difference even if i do the control first and my treatment group later for MTS assay (he also suggest me to do so). I doubt whether what they say is corrected and acceptable in term of methodology.
Furthermore, in MTS, the experiment should carry out for triplicate and repeat 3 times. I would like to know whether the timeframe of first experiment can slightly overlap with second one. For example, may i start my first MTS experiment at day 1 and start second MTS experiment at day 3 even through first MTS experiment is still going on?
Posted 24 November 2010 - 02:55 PM
Posted 25 November 2010 - 04:13 PM