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Nested PCR/ Agarose gel

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#1 George250



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Posted 24 November 2010 - 03:37 AM

Hey guys,

I need your advice please. I am doing for first time methylation analysis that involves nested pcr. I ordered primers (for 1st round and 2nd round of nested) and I would like to run a gel to confirm the presence of the amplified product. Although, the company that I got the primers didnt mention the bp. What do I do in this case? If I see a lanes in my gel it means its OK regardless the size?

Thanx for your time!
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