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Backwards electrophoresis electrodes

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#1 seanspotatobusiness



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Posted 23 November 2010 - 07:18 AM

I put my gel in the electrophoresis container the wrong way around, so to rectify, I switched the connections around. I was wondering whether that might damage the electrodes? Are they made from two different things or are they both the same material? I think in some applications, the anode is a different material from the cathode, but I don't know about this one.

#2 mdfenko


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Posted 23 November 2010 - 07:38 AM

electrophoresis electrodes are usually platinum wires. switching will cause no harm and is required for some methods (eg: acid pH native page).

some electrotransfer apparati (most notably semidry) use different electrode materials for anodes and cathodes but the occasional reversal should not have a significant negative affect on them.
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